T. Uchida T",hasbullah,"nakamura et al., CROSS-REACTIVITY OF SERUM ANTIBODIES FROM CHICKENS IMMUNIZED WITH 3 EIMERIAN SPECIES, Journal of veterinary medical science, 56(5), 1994, pp. 1021-1023
Cross reactivity of serum antibodies from chicken immunized with E. te
nella, E. necatrix and E. acervulina was observed. The enzyme linked i
mmunosorbent assay (ELISA) using soluble antigen prepared from oocysts
of each species showed the cross reactivity among chicken serum immun
ized with the three species of protozoa, especially between E. tenella
and E. necatrix. In immunoblot analysis with E. tenella oocyst antige
n, 7 major bands were detected by homologous E. tenella antiserum and
10 and 6 bands were reacted by E. necatrix and E. acervulina antiserum
respectively. In these antigens, the band of 33 kDa was regarded as c
ross reactive agent between E. tenella and E. necatrix because of high
reactivity to both of the antiserum. In addition, the 130 kDa antigen
that reacted with every antiserum was regarded as common antigen amon
g these three species.