ANALYSIS OF MONOCLONAL-ANTIBODIES REACTIVE WITH THE PORCINE CD4 ANTIGEN

As result of the First International Swine CD Workshop, four monoclona l antibodies (mAb) (#002, 054, 118, and 127) clustered closely to the internal standard anti-porcine CD4 mAb, 74-12-4. To further characteri ze the relationship between these mAb, they were used in flow cytometr y to inhibit binding of 74-12-4 to porcine lymphocytes. mAb #002 (74-1 2-4) and #054 (PT90) completely inhibited, while mAb #127 (10.2H2) and #118 (b38c6) partially inhibited (57% and 77% respectively), the bind ing of 74-12-4. Furthermore, none of the mAbs bound to a 74-12-4 negat ive strain of pigs. We conclude that the four mAb bind to the same, or a closely related, epitope on porcine lymphocytes.