Gj. Tsay et al., SS-A RO ANTIBODIES IN CHINESE PATIENTS WITH SYSTEMIC LUPUS-ERYTHEMATOSUS AND SJOGRENS-SYNDROME/, Journal of the Formosan Medical Association, 95(12), 1996, pp. 905-910
Serum from 53 patients with systemic lupus erythematosus (SLE) and 23
patients with primary Sjogren's syndrome (SS) were studied for anti-52
-kDa SS-A/ Ro, anti-60-kDa SS-A/Ro, and anti-SS-B/La antibodies bg imm
unoblotting and enzyme-linked immunosorbent assay (ELISA). By immunobl
otting, anti-SS-A/Ro was detected in 16 (30%) patients with SLE and 17
(74%) patients with SS. Anti-SS-B/La was detected in 22 (41%) patient
s with SLE and 15 (65%) patients with SS. Serum from 14 of the 16 SLE
patients with anti-SS-A/Ro reacted with the 60-kDa protein and 15 seru
m samples from these patients recognized the 52-kDa protein. Serum wit
h anti-60-kDa SS-A/Ro alone was not found. Serum from all of the 17 SS
patients with anti-SS-A/Ro reacted with the 52-kDa protein, whereas s
erum from only two of these patients recognized the 60-kDa protein. By
ELISA, the frequency of anti-SS-A/Ro (antibodies to the 60-kDa and/or
52-kDa of SS-A/Ro proteins) in patients with SLE and SS was 43/53 (81
%) and 15/23 (65%), respectively. Anti-48-kDa SS-B/La was found in 28%
and 48% of SLE and SS patients, respectively. Serum from 77% of SLE p
atients and 48% of SS patients reacted with the 60-kDa SS-A/Ro protein
. Serum from 45% of SLE patients and 52% of SS patients reacted with t
he 52-kDa SS-A/Ro protein. Patients with SLE had significantly higher
titers of antibodies to 60-kDa SS-A/Ro compared with patients with SS.
Anti-SS-A/Ro and anti-SS-B/La are common in both SLE and SS. The diff
erent reactivities of anti-52-kDa and anti-60-kDa antibodies in serum
from patients with SLE and SS may represent differences in conformatio
n-dependent epitopes of SS-A/Ro autoantigens.