DEMONSTRATION OF CYCLIN D1 (BCL-L) IN MANTLE CELL LYMPHOMA - ENHANCEDSTAINING USING HEAT AND ULTRASOUND EPITOPE RETRIEVAL

Authors
BRYNES RK MCCOURTY A TAMAYO R JENKINS K BATTIFORA H
Citation
Rk. Brynes et al., DEMONSTRATION OF CYCLIN D1 (BCL-L) IN MANTLE CELL LYMPHOMA - ENHANCEDSTAINING USING HEAT AND ULTRASOUND EPITOPE RETRIEVAL, Applied immunohistochemistry, 5(1), 1997, pp. 45-48
Citations number
8
Categorie Soggetti
Immunology
Journal title
Applied immunohistochemistry → ACNP
ISSN journal
10623345
Volume
5
Issue
1
Year of publication
1997
Pages
45 - 48
Database
ISI
SICI code
1062-3345(1997)5:1<45:DOCD(I>2.0.ZU;2-X
Abstract
Cyclin D1/bcl-1 is overexpressed in mantle cell lymphoma. Demonstratio n of this nucleoprotein by immunohistochemistry may be diagnostically useful. We evaluated the recently described sonication-induced method of epitope retrieval and compared it with microwave heat-induced epito pe unmasking. The strongest staining intensities and lowest background staining were achieved when both epitope unmasking methods were combi ned.