POSTTRANSCRIPTIONAL SILENCING OF REPORTER TRANSGENES IN TOBACCO CORRELATES WITH DNA METHYLATION

Authors
INGELBRECHT I VANHOUDT H VANMONTAGU M DEPICKER A
Citation
I. Ingelbrecht et al., POSTTRANSCRIPTIONAL SILENCING OF REPORTER TRANSGENES IN TOBACCO CORRELATES WITH DNA METHYLATION, Proceedings of the National Academy of Sciences of the United Statesof America, 91(22), 1994, pp. 10502-10506
Citations number
51
Categorie Soggetti
Multidisciplinary Sciences
Journal title
Proceedings of the National Academy of Sciences of the United Statesof America → ACNP
ISSN journal
00278424
Volume
91
Issue
22
Year of publication
1994
Pages
10502 - 10506
Database
ISI
SICI code
0027-8424(1994)91:22<10502:PSORTI>2.0.ZU;2-K
Abstract
Endogenous plant genes or transgenes can be silenced on introduction o f homologous gene sequences. Here we document a reporter gene-silencin g event in Nicotiana tabacum that has a distinctive combination of fea tures-i.e., (i) silencing occurs by a posttranscriptional process, (ii ) silencing correlates with DNA methylation, and (iii) this de novo me thylation is not restricted to cytosines located in the symmetrical mo ths CG and CXG.