Sl. Abidi et al., SEPARATIONS OF MAJOR SOYBEAN PHOSPHOLIPIDS ON BETA-CYCLODEXTRIN-BONDED SILICA, Journal of liquid chromatography, 17(17), 1994, pp. 3705-3725
The four major phospholipids (PL) phosphatidylcholine (PC), phosphatid
ylethanolamine (PE), phosphatidylinositol (PI), phosphatidic acid (PA)
found in soybean oil were separated by normal-phase high-performance
liquid chromatography (HPLC) on beta-cyclodextrin-bonded silica (CDS)
with UV detection. Adequate base-line separations of the PL components
were achieved by isocratic elution with mobile phases containing hexa
ne, isopropanol, ethanol and water/tetramethylammonium phosphate (TMAP
). The presence of TMAP in the mobile phases was critical to improve c
omponent resolution and enhance peak symmetry. Analyte retention and c
omponent separations were dramatically influenced by a small change in
mobile phase compositions. Under the HPLC conditions employed, the el
ution order appeared to follow Dhe order of increasing PL polarity wit
h increasing retention times (PE < PC < PI < PA). The HPLC method was
used in the qualitative analyses of selected commercial lecithin sampl
es. Potential applicability of the HPLC-W-CDS technique for the quanti
tative analysis of several crude oil samples derived from genetically
modified soybean oil is demonstrated.