Ejm. Geertman et al., DEGRADATION OF L-5-VINYLOXAZOLIDINE-2-THIONE, A GOITROGENIC PRODUCT FROM RAPESEED, BY RUMEN MICROORGANISMS, Journal of environmental science and health. Part A: Environmental science and engineering, 29(9), 1994, pp. 2009-2021
The use of rapeseed meal for animal feeding is limited by the presence
of antinutritional compounds, such as glucosinolates. An in vitro rum
en system was used to test the effect of L-5-vinyloxazolidine-2-thione
(VOT), a toxic hydrolysis product from glucosinolates found in rapese
ed. The effect of VOT on rumen performance was minimal. The VOT was de
graded efficiently (60-70%) in the artificial rumen system, when rapes
eed was fed batchwise at 20% (w/w) of the diet. The degradation of VOT
is caused by bacteria because the addition of the (oil containing) ra
peseed resulted in a disappearance of ciliated protozoa. Experiments w
ith pure VOT resulted in complete degradation both in batch (2 mM) and
in continuous culture (4 mM).