A. Vershinin et al., CHARACTERIZATION OF A FAMILY OF TANDEMLY REPEATED DNA-SEQUENCES IN TRITICEAE, Theoretical and Applied Genetics, 89(2-3), 1994, pp. 217-225
The recombinant plasmid dpTa1 has an insert of relic wheat DNA that re
presents a family of tandemly organized DNA sequences with a monomeric
length of approximately 340 bp. This insert was used to investigate t
he structural organization of this element in the genomes of 58 specie
s within the tribe Triticeae and in 7 species representing other tribe
s of the Poaceae. The main characteristic of the genomic organization
of dpTa1 is a classical ladder-type pattern which is typical for tande
mly organized sequences. The dpTa1 sequence is present in all of the g
enomes of the Triticeae species examined and in 1 species from a close
ly related tribe (Bromus inermis, Bromeae). DNA from Hordelymus europa
eus (Triticeae) did not hybridize under the standard conditions used i
n this study. Prolonged exposure was necessary to obtain a weak signal
. Our data suggest that the dpTa1 family is quite old in evolutionary
terms, probably more ancient than the tribe Triticeae. The dpTa1 seque
nce is more abundant in the D-genome of wheat than in other genomes in
Triticeae. DNA from several species also have bands in addition to th
e tandem repeats. The dpTa1 sequence contains short direct and inverte
d subrepeats and is homologous to a tandemly repeated DNA sequence fro
m Hordeum chilense.