DETERMINATION OF THE ENANTIOMERS OF BUNOLOL IN HUMAN URINE BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY ON A CHIRAL AGP STATIONARY-PHASE AND IDENTIFICATION OF THEIR METABOLITES BY GAS-CHROMATOGRAPHY MASS-SPECTROMETRY

A high-performance liquid chromatographic method using a chiral AGP co lumn was developed to screen and determine the enantiomers of bunolol in human urine. The recovery of (+)- and (-)-bunolol from urine was 91 .79-95.23% at different concentrations. The coefficients of variation (C.V.) were less than 2.1 and 2.3% for intra- and inter-assays, respec tively. Urinary metabolites were detected using GC-MS after derivatiza tion with N-methyl(trimethylsilyI)trifluroacetamide. The influences of pH and modifier on a chiral AGP column were studied.