PROCEDURES FOR SXS ANTIGEN-DETECTION BY ANTIBODY-MEDIATED CYTOTOXICITY TESTS - A COMPARATIVE-ANALYSIS

Biological reagents used in the serological detection of Sxs antigen b y antibody-mediated cytotoxicity tests were compared in order to optim ize the method. Our analyses showed that: (a) red cell-free spleen cel ls are the best target cells, (b) rabbit serum used as the complement source should be obtained from females, and absorbed with female splee n cells before use, (c) antiserum obtained by immunizing females with repeated injections of syngenic male spleen cells provides the highest anti-Sxs antibody titer, and (d) of the different biological fluids i nvestigated, testis supernatant has highest concentration of Sxs antig en.