Nj. Moore et Ab. Margolin, EFFICACY OF NUCLEIC-ACID PROBES FOR DETECTION OF POLIOVIRUS IN WATER DISINFECTED BY CHLORINE, CHLORINE DIOXIDE, OZONE, AND UV-RADIATION, Applied and environmental microbiology, 60(11), 1994, pp. 4189-4191
MilliQ water was inoculated with poliovirus type 1 strain LSc-1 and wa
s treated with disinfectants, including chlorine, chlorine dioxide, oz
one, and UV light. No relationship between probes and plaque assays we
re seen, demonstrating that viral nucleic acids were not destroyed. Th
ese findings suggest that nucleic acid probes cannot distinguish betwe
en infectious and noninfectious viruses and cannot be used in the eval
uation of treated waters.