CONSTRUCTION OF AGROBACTERIUM STRAINS BY ELECTROPORATION OF GENOMIC DNA AND ITS UTILITY IN ANALYSIS OF CHROMOSOMAL VIRULENCE MUTATIONS

Authors
CHARLES TC DOTY SL NESTER EW
Citation
Tc. Charles et al., CONSTRUCTION OF AGROBACTERIUM STRAINS BY ELECTROPORATION OF GENOMIC DNA AND ITS UTILITY IN ANALYSIS OF CHROMOSOMAL VIRULENCE MUTATIONS, Applied and environmental microbiology, 60(11), 1994, pp. 4192-4194
Citations number
22
Categorie Soggetti
Microbiology,"Biothechnology & Applied Migrobiology
Journal title
Applied and environmental microbiology → ACNP
ISSN journal
00992240
Volume
60
Issue
11
Year of publication
1994
Pages
4192 - 4194
Database
ISI
SICI code
0099-2240(1994)60:11<4192:COASBE>2.0.ZU;2-X
Abstract
We have extended the technique of electroporation as a genetic tool fo r manipulating the Agrobacterium tumefaciens chromosome. We used this technique to introduce chromosomal DNA into recipient A. tumefaciens s trains by electroporation and constructed isogenic chvE mutants that s hare the same chromosomal background but differ in their types of pTi (octopine or nopaline). Both nopaline and octopine pTi-carrying chvE m utants were deficient in vir regulon induction and exhibited similar r eductions in host range.