ALTERATION OF SUBSTRATE-SPECIFICITY AND OPTIMUM PH OF SARCOSINE OXIDASE BY RANDOM AND SITE-DIRECTED MUTAGENESIS

Authors
NISHIYA Y IMANAKA T
Citation
Y. Nishiya et T. Imanaka, ALTERATION OF SUBSTRATE-SPECIFICITY AND OPTIMUM PH OF SARCOSINE OXIDASE BY RANDOM AND SITE-DIRECTED MUTAGENESIS, Applied and environmental microbiology, 60(11), 1994, pp. 4213-4215
Citations number
8
Categorie Soggetti
Microbiology,"Biothechnology & Applied Migrobiology
Journal title
Applied and environmental microbiology → ACNP
ISSN journal
00992240
Volume
60
Issue
11
Year of publication
1994
Pages
4213 - 4215
Database
ISI
SICI code
0099-2240(1994)60:11<4213:AOSAOP>2.0.ZU;2-A
Abstract
The sarcosine oxidase gene was randomly mutated, and F103V, which show ed altered specificity, was screened with the plate assay. Five differ ent mutants at position 103 were also constructed. Each mutant exhibit ed a rather striking change in substrate specificity, except for F103W . The optimum pH was also shifted, depending on the substrate.