ENZYMATIC COUPLING OF ALPHA,ALPHA-DIALKYL AMINO-ACIDS USING INVERSE SUBSTRATES AS ACYL DONORS

Authors
SEKIZAKI H ITOH K TOYOTA E TANIZAWA K
Citation
H. Sekizaki et al., ENZYMATIC COUPLING OF ALPHA,ALPHA-DIALKYL AMINO-ACIDS USING INVERSE SUBSTRATES AS ACYL DONORS, Tetrahedron letters, 38(10), 1997, pp. 1777-1780
Citations number
16
Categorie Soggetti
Chemistry Inorganic & Nuclear
Journal title
Tetrahedron letters → ACNP
ISSN journal
00404039
Volume
38
Issue
10
Year of publication
1997
Pages
1777 - 1780
Database
ISI
SICI code
0040-4039(1997)38:10<1777:ECOAAU>2.0.ZU;2-7
Abstract
Two series of inverse substrates, N-alpha-Boc-alpha,alpha-dialkyl amin o acid p-guanidino- and p-(guanidinomethyl)phenyl esters, were prepare d as acyl donor components for enzymatic peptide synthesis. They were found to be readily coupled with amino acid p-nitroanilide to produce peptide. Streptomyces griseus trypsin was a more efficient catalyst th an bovine trypsin. (C) 1997 Elsevier Science Ltd.