Jm. Sullivan et al., IDENTIFICATION OF 2 CYSTEINE RESIDUES THAT ARE REQUIRED FOR REDOX MODULATION OF THE NMDA SUBTYPE OF GLUTAMATE-RECEPTOR, Neuron, 13(4), 1994, pp. 929-936
Modulation of NMDA-mediated responses by oxidizing and reducing reagen
ts has been described in a variety of neuronal preparations. Here, we
report that NMDA-gated currents of oocytes expressing heteromeric NMDA
receptors are also modulated by sulfhydryl redox reagents. Each cyste
ine residue in the NMDAR1 (NR1) subunit and each conserved NMDAR2 (NR2
) cysteine residue in a prototypical subunit (NR2B) was tested for its
role in redox modulation. We have identified 2 cysteines in the NR1 s
ubunit that are required for redox modulation of NMDA-gated currents i
n oocytes expressing NR1-NR2B, NR1-NR2C, or NR1-NR2D receptors. Mutati
on of these same 2 cysteines also eliminated potentiation by spermine
and shifted the IC50 for H+ inhibition and the EC(50) for NMDA. Redox
modulation of heteromeric NR1-NR(2)A receptors appeared to be differen
t from that of the other heteromeric receptors, indicating the presenc
e of one or more unique redox modulatory sites on NR1-NR2A receptors.