INCREASE OF INHERITABLE POLYMORPHISMS OF ARBITRARY PRIMED PCR PRODUCTS ON DGGE GELS

Authors
PROCUNIER JD WOLF M HOWES NK
Citation
Jd. Procunier et al., INCREASE OF INHERITABLE POLYMORPHISMS OF ARBITRARY PRIMED PCR PRODUCTS ON DGGE GELS, Biotechnology techniques, 8(10), 1994, pp. 707-710
Citations number
7
Categorie Soggetti
Biothechnology & Applied Migrobiology
Journal title
Biotechnology techniques → ACNP
ISSN journal
0951208X
Volume
8
Issue
10
Year of publication
1994
Pages
707 - 710
Database
ISI
SICI code
0951-208X(1994)8:10<707:IOIPOA>2.0.ZU;2-8
Abstract
Single 10-mer primers of arbitrary sequence were used to amplify, by p olymerase chain reaction (PCR), random genomic regions of two closely related hexaploid wheat cultivars. Polymorphic bands between the two g enomes were visualized on both agarose and denaturing gradient gel ele ctrophoresis (DGGE) gels. There was approximately a four-fold increase in inheritable polymorphisms when the PCR fragments were separated on DGGE gels.