PHENYLALANINE AMMONIA-LYASE ACTIVITY IN PERMEABILIZED YEAST-CELLS (RHODOTORULA-GLUTINIS)

Authors
SRINIVASAN AR",NAGAJYOTHI,"GOWDA LR BHAT SG
Citation
Lr. Srinivasan Ar",nagajyothi,"gowda et Sg. Bhat, PHENYLALANINE AMMONIA-LYASE ACTIVITY IN PERMEABILIZED YEAST-CELLS (RHODOTORULA-GLUTINIS), Biotechnology techniques, 8(10), 1994, pp. 729-732
Citations number
5
Categorie Soggetti
Biothechnology & Applied Migrobiology
Journal title
Biotechnology techniques → ACNP
ISSN journal
0951208X
Volume
8
Issue
10
Year of publication
1994
Pages
729 - 732
Database
ISI
SICI code
0951-208X(1994)8:10<729:PAAIPY>2.0.ZU;2-S
Abstract
Whole cells of the yeast Rhodotorula glutinis IFO 0559 had low phenyla lanine ammonia-lyase (PAL) activity due to a limited membrane permeabi lity barrier for phenylalanine. Permeabilization with detergents and o rganic solvents increased cellular PAL activity significantly and of t hese cetyltrimethylammonium bromide gives the maximum increase (6- fol d). PAL activity of such detergent permeabilized cells could be used f or the synthesis and degradation of L-phenylalanine