ENZYMATIC PROFILES OF MYCOBACTERIUM SPP AND THEIR VALUE FOR SPECIES DIFFERENTIATION

Authors
KAMPFER P KROPPENSTEDT RM
Citation
P. Kampfer et Rm. Kroppenstedt, ENZYMATIC PROFILES OF MYCOBACTERIUM SPP AND THEIR VALUE FOR SPECIES DIFFERENTIATION, Journal of General and Applied Microbiology, 40(3), 1994, pp. 209-214
Citations number
18
Categorie Soggetti
Microbiology,"Biothechnology & Applied Migrobiology
Journal title
Journal of General and Applied Microbiology → ACNP
ISSN journal
00221260
Volume
40
Issue
3
Year of publication
1994
Pages
209 - 214
Database
ISI
SICI code
0022-1260(1994)40:3<209:EPOMSA>2.0.ZU;2-I
Abstract
A total of 140 strains of Mycobacterium species were studied for their ability to hydrolyze 54 different fluorogenic 4-methylumbelliferyl (4 -MU)- and 2-naphthylamide (2-NA)-linked fluorogenic substrates within 6 and 24 h of incubation. Enzymatic profiles were heterogeneous within the genus, and some of the tests can improve the differentiation of M ycobacterium species. However, enzymatic profiles are not sufficient f or unambiguous identification and should be used in combination with o ther biochemical tests.