TISSUE CONTENT OF ALPHA-TOCOPHEROL AND OXIDATIVE STABILITY OF BROILERS RECEIVING DIETARY ALPHA-TOCOPHERYL ACETATE SUPPLEMENT FOR VARIOUS PERIODS PRE-SLAUGHTER

1. The effects of dietary alpha-tocopheryl acetate on the alpha-tocoph erol status of chicken plasma and tissues were investigated. The rate of iron-ascorbate-induced lipid peroxidation was also studied. 2. One hundred and forty four chicks were divided into 6 groups: one control group was fed a basal diet of 30 mg alpha-tocopheryl acetate/kg food f or the duration of the trial. A supplemental diet of 200 mg alpha-toco pheryl acetate was fed to each of the other 5 groups for 1,2,3,4 or 5 weeks prior to slaughter.3. Supplementation resulted in an increase in alpha-tocopherol in plasma and all tissues examined. Saturation level s of alpha-tocopherol were observed in plasma after 1 week of feeding and in tissues within 3 to 4 weeks of feeding. 4. Supplementation with alpha-tocopheryl acetate for up to 4 weeks pre-slaughter resulted in significant reductions in susceptibility to induced lipid peroxidation . 5. Overall, the results show that feeding 200 mg alpha-tocopherol ac etate/kg food to chicks for at least 4 weeks prior to slaughter is nec essary to optimise muscle content and stability against lipid peroxida tion.