H. Tsuruga et al., APPLICATION OF DNA-FINGERPRINTING IN THE HOKKAIDO BROWN BEAR (URSUS-ARCTOS YESOENSIS), Journal of veterinary medical science, 56(5), 1994, pp. 887-890
DNA fingerprinting employing a minisatellite Myo probe was used for in
dividual identification and paternity determination in Hokkaido brown
bears (Ursus arctos yesoensis). We used two restriction enzymes, HinfI
and HaeIII to make DNA fingerprints. Band patterns obtained from rand
omly selected bears were compared with each other, and the probability
x that fragment in an individual was also present in the other was 0.
69 for HinfI and 0.83 for HaeIII. The value for HinfI (0.69) was simil
ar to that obtained from other species, such as dog and domestic anima
ls, and the mean probability of all fragments was calculated to be 2.5
x 10(-2). The results suggest that DNA fingerprinting applying the co
mbination of HinfI and Myo is available for individual identification.
On the other hand, the ability to determine paternity seemed to be in
sufficient owing to the lack of paternal fragments, although the band
patterns reflected the correct relationships between child and father.