RECOGNITION OF MONOSIALOGANGLIOSIDE (GM(3)) RECONSTITUTED IN SPHINGOMYELIN AND GLUCOSYLCERAMIDE MEMBRANES AGAINST WHEAT-GERM-AGGLUTININ - QUANTITATIVE-ANALYSES BY A QUARTZ-CRYSTAL MICROBALANCE

Specific binding of Wheat Germ Agglutinin (WGA) to GM(3)-containing mi xed monolayer at the air-water interface was studied by using a quartz crystal microbalance to know the binding amount and initial binding r ate of WGA to GM(3). GM(3) in the glucosylceramide matrix membrane sho wed the high recognizability compared to GM(3) in sphingomyeline matri x membrane. It is demonstrated that matrix lipids surrounding GM(3) re gulated the recognition of GM(3). (C) 1994 Academic Press, Inc.