THE TUMOR PROTEIN MAGE-1 IS LOCATED IN THE CYTOSOL OF HUMAN-MELANOMA CELLS

Authors
AMARCOSTESEC A GODELAINE D STOCKERT E VANDERBRUGGEN P BEAUFAY H CHEN YT
Citation
A. Amarcostesec et al., THE TUMOR PROTEIN MAGE-1 IS LOCATED IN THE CYTOSOL OF HUMAN-MELANOMA CELLS, Biochemical and biophysical research communications, 204(2), 1994, pp. 710-715
Citations number
14
Categorie Soggetti
Biology,Biophysics
Journal title
Biochemical and biophysical research communications → ACNP
ISSN journal
0006291X
Volume
204
Issue
2
Year of publication
1994
Pages
710 - 715
Database
ISI
SICI code
0006-291X(1994)204:2<710:TTPMIL>2.0.ZU;2-R
Abstract
The MAGE-1 protein has been localized to the cytosol of human melanoma culture cells (MZ2-MEL.43) by subcellular fractionation. Its distribu tion between nuclear, mitochondrial, microsomal and cytosolic fraction s was established by SDS-PAGE and immunoblotting with a rabbit antiser um and compared to that of markers for the main cell compartments. The immunoreactive material was recovered in the cytosolic fraction as a polypeptide migrating at 42 kDa, which has been further identified as the MAGE-1 protein because it comigrates with the product of cell-free transcription-translation of the MAGE-1 cDNA. Location to the cytosol was confirmed by immunofluorescence microscopy. (C) 1994 Academic Pre ss, Inc.