GLUCOSE-6-PHOSPHATASE GENE-EXPRESSION AND ACTIVITY ARE MODULATED IN HEMORRHAGIC-SHOCK - EVIDENCE FOR A NEW HEAT-SENSITIVE ACTIVATOR

Decreased hepatic fructose 2,6-bisphosphate levels were observed in th e early phase of hemorrhagic shock. The lower sugar bisphosphatae leve l was a result of increased phosphoenolpyruvate levels and decreased g lucose-6-phosphate and fructose-6-phosphate levels. The decreased gluc ose-6-phosphate levels correlated with increased activity of liver glu cose-6-phosphatase and a concomitant 2.5-fold increase in glucose-6-ph oaphatase mRNA abundance. In addition, protein-free filtrate from hemo rrhagic shock rats, but not from control rats, increased glucose-6-pho sphatase activity. However, when control and hemorrhagic shock protein -free filtrates wars heated, they both increased the glucose-6-phospha tase activity of the respective microsomes to the same extent. It is c oncluded that the early hyperglycemic phase of hemorrhagic shock is du e to enhanced glucose-6-phosphatase gene expression and activity and t he generation of a heat sensitive activator of the enzyme. (C) 1994 Ac ademic Press, Inc.