R. Tachezy et al., ANALYSIS OF SHORT NOVEL HUMAN PAPILLOMAVIRUS SEQUENCES, Biochemical and biophysical research communications, 204(2), 1994, pp. 820-827
Consensus primer mediated PCR protocols have the potential to amplify
previously uncharacterized human papillomavirus (EBV) genotypes. In a
study on 500 cervicovaginal samples, we amplified four sequences (L1AE
1 to L1AE 4) that failed to hybridize to any of the available HPV typ
e-specific oligonucleotide probes. Nucleotide sequencing revealed that
the sequences were derived from the L1 region of hitherto unsequenced
genotypes. Comparison of phylogenetic trees based on the amplified L1
sequences with E6-derived phylogenetic trees resulted in the identifi
cation of L1AE 1 and L1AE 2 as putative novel HPV PCR genotypes. L1AE
1 was related to HPV 39, whereas L1AE 2 was related to HPV 51. The L1A
E 3 and L1AE 4 sequences occupied L1-phylogenetic branches equivalent
to the positions of HPV 66 and HPV 61, respectively, in an E6-phylogen
etic tree. (C) 1994 Academic Press, Inc.