GLYCANS AS TARGETS FOR MONOCLONAL-ANTIBODIES THAT PROTECT RATS AGAINST TRICHINELLA-SPIRALIS

Citation
La. Ellis et al., GLYCANS AS TARGETS FOR MONOCLONAL-ANTIBODIES THAT PROTECT RATS AGAINST TRICHINELLA-SPIRALIS, Glycobiology, 4(5), 1994, pp. 585-592
Citations number
31
Categorie Soggetti
Biology
Journal title
ISSN journal
09596658
Volume
4
Issue
5
Year of publication
1994
Pages
585 - 592
Database
ISI
SICI code
0959-6658(1994)4:5<585:GATFMT>2.0.ZU;2-#
Abstract
We have investigated the role of glycans on Trichinella spiralis antig ens in recognition by rat monoclonal antibodies (mAbs) which protect r at pups against challenge with the parasite. In pups born to infected dams or pups passively immunized with mAbs, antibodies eliminate a cha llenge dose from the intestine within hours ((rapid expulsion'). Becau se such dramatic protection can be afforded by mAbs, we have sought to characterize the parasite antigens they target. In this report we sho w that protective antibodies were unable to bind excretory/secretory ( ES) antigens deglycosylated with trifluoromethanesulphonic acid (TFMS) . In addition, oligosaccharides isolated from glycoproteins by alkalin e hydrolysis or peptide: N glycosidase F (PNGase F) digestion were bou nd by protective, but not non-protective, mAbs. Glycans affinity purif ied with protective mAb 9D bound to all but one protective mAb. These antibodies have been shown previously to bind to the surfaces of intac t larvae, indicating that the glycan is exposed on the parasite surfac e. Candidate glycans that may be involved in binding protective mAbs h ave unusual tri- and tetra-antennary structures with terminal tyvelose moieties (Reason et al., Glycobiology, 4, 000-000, 1994). Coating of the larval surface with such glycans may serve to protect the parasite and its secreted products from enzymatic attack as the parasite trave ls to and resides in its epithelial niche.