CROSS-COLUMN RETENTION PREDICTION IN REVERSED-PHASE LIQUID-CHROMATOGRAPHY USING FACTOR ANALYTICAL MODELING

It is proposed here that the relative solvent strength relationship mo ly be transferable between similar columns (e.g., C-18 packing). The t ransfer between columns may be realized by using a ''key set'' solvent -projection matrix derived from measurements on one column and measure ments for compounds in a key solvent set on each new column. This hypo thesis is tested from water/methanol/acetonitrile solvent systems usin g data collected on three different C-18 reversed-phase columns. The a verage crossing prediction errors, ranging from 2% to 5% based on capa city factor, are obtained for various combinations of key solvent and prediction sets.