REACTIVITY OF MONOCLONAL-ANTIBODIES TO POTATO-VIRUS-A IN VARIOUS TYPES OF ENZYME-LINKED-IMMUNOSORBENT-ASSAY

A panel of mouse monoclonal antibodies (MAbs) was prepared against pot ato virus A (PVA) and their reactivity was tested in various types of ELISA. The type of ELISA as well as the methods of MAbs purification p layed an essential role. All monoclonal antibodies reacted with PVA an tigen in IDAS-ELISA but MAbs used in DAS(1)-ELISA and in DAS(2)-ELISA, in combination with polyclonal antibodies, showed different reactivit y. The reactivity of MAbs in DAS-ELISA was dependent on the individual MAb used as a coating or as a conjugate. Two MAbs showed highest reac tivity as coating antibodies but only one of them as a conjugate. The results demonstrate the importance of using different type of ELISA in selecting MAbs for routine diagnosis.