CHEMOMETRIC CLASSIFICATION OF L-TRYPTOPHAN LOTS FROM GENETICALLY-MODIFIED BACILLUS-AMYLOLIQUEFACIENS STRAINS

Four types of L-tryptophan lots produced by genetically modified B. my loliquefaciens strains II-V, which consisted of case-associated (11) w ith manifestations of EMS, control (16), and other lots (11), were com pared by multivariate computer recognition programs (chemometrics) inc luding HCA, PCA, KNN and SIMCA, as well as PLS. In HCA data analysis l ots from strains III and V formed discrete clusters, and in PCA and SI MCA, both lots formed separated groups. Lots from strains II and TV we re included in a group of lots from strain III. This indicated strains II and IV too were closely related to strain III. When strain vs case -associated control or other lots were compared by four methods, SIMCA provided adequate strain separation and strains were well grouped in case-associated or control lots.