COATED-BEAD ASSAY FOR MICROBIAL CELL-ASSOCIATED AND EXTRACELLULAR PROTEASE ACTIVITY

Authors
ASCENCIO F WADSTROM T
Citation
F. Ascencio et T. Wadstrom, COATED-BEAD ASSAY FOR MICROBIAL CELL-ASSOCIATED AND EXTRACELLULAR PROTEASE ACTIVITY, Microbios, 79(320), 1994, pp. 139-144
Citations number
16
Categorie Soggetti
Microbiology
Journal title
Microbios → ACNP
ISSN journal
00262633
Volume
79
Issue
320
Year of publication
1994
Pages
139 - 144
Database
ISI
SICI code
0026-2633(1994)79:320<139:CAFMCA>2.0.ZU;2-8
Abstract
An assay utilizing latex beads coated with substrates of (125)l-labell ed connective tissue proteins is described as an alternative method fo r quantitating cell-associated and extracellular proteolytic activity of micro-organisms. Proteolytic activity is a function of the amount o f (125)l-labelled peptides released into the incubation mixture.