Mc. Pietrogrande et Yd. Kahie, EFFECT OF THE MOBILE AND STATIONARY PHASES ON RP-HPLC RETENTION AND SELECTIVITY OF FLAVONOID COMPOUNDS, Journal of liquid chromatography, 17(17), 1994, pp. 3655-3670
The retention behaviour of an extended set of flavonoid compounds on p
henyl and cyano reversed-phase HPLC columns was studied and compared t
o behaviour on octadecyl column. The selectivity properties of methano
l, acetonitrile and tetrahydrofuran as organic modifiers on each stati
onary phase are reported. Both the stationary and the mobile phases ap
peared to significantly change HPLC system selectivity. Specific stati
onary phase selectivity effects proved more pronounced with methanol:
in particular the phenyl phase showed a greater selective retention fo
r unsaturated flavonoids while octadecyl proved more selective for gly
coside compounds.