CHARACTERIZATION OF MICROSATELLITES FROM FLOW-SORTED PORCINE CHROMOSOME-13

Porcine flow-sorted Chromosome (Chr) 13 was PCR amplified with primers based on porcine short interspersed element (SINE) sequences. The pro duct was cloned, gridded in microtiter plates, and screened with a [GT ](10) oligonucleotide which gave 45 positive clones. Sequencing of the se clones showed that 36 were unique, and 26 [GT](n) microsatellites w ere characterized. Six other simple repeat sequences, the majority of which were associated with the 3' end of the SINE sequence, were also detected. Twenty-one primers sets were selected, and 13 of these detec ted useful polymorphisms in the grandparents (n = 26) of the European porcine mapping collaboration (PiGMaP) reference families. These 13 ma rkers were mapped in the ''PiGMaP'' reference families, and a two-poin t linkage analysis was performed. The Lod scores indicated that three of the markers were not linked and the remaining 11 formed two linkage groups of two and nine markers respectively. The larger linkage group was also linked to the transferrin locus, permitting assignment of ni ne markers to porcine Chr 13.