MECHANOCHEMICAL COMPLEXATION OF MICRONIZED CELLULOSE WITH CO(3+) IONSBY ULTRASONIC TREATMENT IN ORDER TO OBTAIN MACROMOLECULAR CARRIERS FOR INVERTASE IMMOBILIZATION

The paper deals with the study of the mechanochemical complexation of micronized cellulose with Co3+ ions by an ultrasonic treatment. The mi cronized material was obtained from dissolving pulp, using a hydrolyti c process. The complexes were used as macromolecular carriers for inve rtase immobilization. Carrying out the experiments by using a second o rder, centrated, composite, rotatable design, regression equations cor relating the Co3+ content in the complexes and the efficiency of inver tase coupling with the parameters of the ultrasonic process ([Co3+] in the initial solution, temperature and time) were obtained. It was als o studied the influence of the coupling reaction parameteres (enzyme/s upport ratio, time) on the coupling efficiency.