ALFALFA EMBRYO PRODUCTION IN AIRLIFT VESSELS VIA DIRECT SOMATIC EMBRYOGENESIS

Authors
KUKLIN AI DENCHEV PD ATANASSOV AI SCRAGG AH
Citation
Ai. Kuklin et al., ALFALFA EMBRYO PRODUCTION IN AIRLIFT VESSELS VIA DIRECT SOMATIC EMBRYOGENESIS, Plant cell, tissue and organ culture, 38(1), 1994, pp. 19-23
Citations number
23
Categorie Soggetti
Plant Sciences
Journal title
Plant cell, tissue and organ culture → ACNP
ISSN journal
01676857
Volume
38
Issue
1
Year of publication
1994
Pages
19 - 23
Database
ISI
SICI code
0167-6857(1994)38:1<19:AEPIAV>2.0.ZU;2-W
Abstract
A procedure for the development of alfalfa (Medicago falcata L.) somat ic embryos to the torpedo stage in airlift vessels is described. Embry os were initiated from chopped leaf explants and were formed by direct somatic embryogensis. The system produced a high number of torpedo st age embryos. The effect of various inoculation densities on embryo dev elopment was studied. A procedure for the development and maturation o f embryos in aerated liquid media was established. The rate of convers ion of the torpedo stage embryos formed in the vessels was 83%.