Pre-globular stage embryo masses from hormone-free liquid suspension c
ultures of pecan (Carya illinoinensis Koch) have been induced to devel
op into somatic embryos (SE) on semi-solid medium; however, plant reco
very from these embryos has been poor. Lack of storage reserve accumul
ation may be a critical factor. In this study, the effect of modified
solid media treatments on somatic embryo storage reserve accumulation
was appraised. Media were modified with various combinations of abscis
ic acid, maltose, casein hydrolysate, and filter paper overlays. Embry
os were assessed for triglyceride and protein accumulation, compared w
ith levels found within zygotic embryos. High concentrations of maltos
e with sucrose generated reserves in the somatic embryos more closely
approaching mature zygotic embryos. Although some treatments stimulate
d significant reserve deposition, this was associated with unacceptabl
e degeneration in embryo morphology. In general, embryos were repetiti
ve (perpetually undergoing renewed rounds of embryogenesis) while on t
he various media. Reserve-enhancing treatments were identified which m
ay benefit plant recovery from suspension-derived pecan somatic embryo
s.