2-DIMENSIONAL DEMONSTRATION OF MYENTERIC NERVE PLEXUS - APPLICATION FOR PSEUDO-HIRSCHSPRUNGS-DISEASE

The use of two dimensional demonstration of myenteric nerve plexus for the morphological estimation of pseudo Hirschsprung's disease is intr oduced. By fluorescent immunohistochemistry for S-100 protein on flat- mounted frozen sections, a decrease in the amount of ganglionic plexus es in the small intestinal segments in cases of pseudo-Hirschsprung's disease was able to be distinguished from normoganglionic plexuses in control cases. Moreover, a morphological difference between the two ty pes of cases was clearly demonstrated. This method may he an indispens able tool in the reliable diagnosis of pseudo-Hirschsprung's disease. Copyright (C) 1994 by W.B. Saunders Company