DETERMINATION OF HORSERADISH-PEROXIDASE CONCENTRATION USING THE CHEMILUMINESCENCE OF CYPRIDINA LUCIFERIN ANALOG, YOXYPHENYL)-3,7-DIHYDROIMIDAZO[1,2-A]PYRAZIN-3-ONE

Authors
MITANI M YOKOYAMA Y ICHIKAWA S SAWADA H MATSUMOTO T FUJIMORI K KOSUGI M
Citation
M. Mitani et al., DETERMINATION OF HORSERADISH-PEROXIDASE CONCENTRATION USING THE CHEMILUMINESCENCE OF CYPRIDINA LUCIFERIN ANALOG, YOXYPHENYL)-3,7-DIHYDROIMIDAZO[1,2-A]PYRAZIN-3-ONE, Journal of bioluminescence and chemiluminescence, 9(6), 1994, pp. 355-361
Citations number
19
Categorie Soggetti
Biology
Journal title
Journal of bioluminescence and chemiluminescence → ACNP
ISSN journal
08843996
Volume
9
Issue
6
Year of publication
1994
Pages
355 - 361
Database
ISI
SICI code
0884-3996(1994)9:6<355:DOHCUT>2.0.ZU;2-8
Abstract
The chemiluminescence of the Cypridina luciferin analogue. hoxyphenyl) -3,7-dihydroimidazo[1,2-a]pyrazin-3-one (MCLA) was observed at 462 nm in the presence of horseradish peroxidase(HRP) and the total spectrum of light emitted was found to depend linearly on HRP concentration. Me thods for the determination of HRP concentration using the chemilumine scence was investigated. HRP could be detected in the range from 100 p mol/L to 100 nmol/L under the optimum condition, H2O2 (10 mmol/L) and MCLA (10 mu mol/L) at pH 5.8.