IN-SITU LOCALIZATION OF THROMBOSPONDIN-1 AND THROMBOSPONDIN-3 TRANSCRIPTS IN THE AVIAN EMBRYO

Citation
Rp. Tucker et al., IN-SITU LOCALIZATION OF THROMBOSPONDIN-1 AND THROMBOSPONDIN-3 TRANSCRIPTS IN THE AVIAN EMBRYO, Developmental dynamics, 208(3), 1997, pp. 326-337
Citations number
27
Categorie Soggetti
Developmental Biology","Anatomy & Morphology
Journal title
ISSN journal
10588388
Volume
208
Issue
3
Year of publication
1997
Pages
326 - 337
Database
ISI
SICI code
1058-8388(1997)208:3<326:ILOTAT>2.0.ZU;2-#
Abstract
Two novel cDNA probes to chicken thrombospondin-1 (TSP-1) and TSP-3 we re used to determine by in situ hybridization the origins of these ext racellular matrix proteins during embryogenesis. Both TSP-1 and TSP-3 are expressed in embryonic cartilage. TSP-1 expression is limited to e arly chondrocytes, in contrast to TSP-3 mRNAs that are found in older proliferative and hypertrophic chondrocytes. TSP-1 and TSP-3 are expre ssed consecutively during neurogenesis as well, with the TSP-1 probe h ybridizing in proliferating neuroblasts, and TSP-3 expressed by neuron s as they are actively extending processes. A TSP-1 hybridization sign al reappears in subpopulations of neurons in the spinal cord and brain after the periods of active neurite extension and programmed cell dea th are complete. TSP-1 is also expressed in the spinal cord floor plat e, in mesenchyme surrounding the developing paramesonephric duct, at t he tips of growing lung bronchioles, in lens, and in corneal endotheli um. These observations indicate that there is little overlap in the ex pression patterns of TSP-1 and TSP-3 and that they are expressed conse cutively during the development of cartilage and neurons. The pattern of TSP-1 expression in avascular tissues and in the floor plate is con sistent with possible roles for this glycoprotein in regulating angiog enesis and establishing morphogenetic gradients. (C) 1997 Wiley-Liss, Inc.