DIOXETANE CHEMILUMINESCENCE DETECTION IN LIQUID-CHROMATOGRAPHY BASED ON PHOTOSENSITIZED ONLINE GENERATION OF SINGLET MOLECULAR-OXYGEN - A THOROUGH EXAMINATION OF EXPERIMENTAL PARAMETERS AND APPLICATION TO POLYCHLORINATED-BIPHENYLS
Hag. Niederlander et al., DIOXETANE CHEMILUMINESCENCE DETECTION IN LIQUID-CHROMATOGRAPHY BASED ON PHOTOSENSITIZED ONLINE GENERATION OF SINGLET MOLECULAR-OXYGEN - A THOROUGH EXAMINATION OF EXPERIMENTAL PARAMETERS AND APPLICATION TO POLYCHLORINATED-BIPHENYLS, Analytica chimica acta, 297(3), 1994, pp. 349-368
Experimental parameters influencing sensitivity and selectivity in che
miluminescence detection based on photoinduced production of singlet m
olecular oxygen in liquid chromatography, an approach introduced in ea
rlier papers, are extensively studied. Normal-phase liquid chromatogra
phy is applied in addition to reversed-phase chromatography, using pol
ychlorinated biphenyls (PCBs) as analytes. The photochemical reactor p
arameters play the most important role. The chemiluminescence detector
parameters are generally restrained by the required photochemical rea
ctor conditions. An alternative, commercially available, reagent for a
ddition of singlet molecular oxygen (i.e., ethyl vinyl ether) and a ne
w photochemical reactor construction are introduced and compared to th
e former ones. The experiments show that the optimum conditions are st
rongly determined by the individual PCB concerned. Planar PCBs known f
or their high toxicity, such as the PCBs 77, 126 and 169 can be detect
ed far more sensitively than the non-planar ones. The inherent selecti
vity of the method is shown for a herring oil sample, spiked with the
above PCBs, utilizing normal-phase liquid chromatography with a pyreny
l column.