Jnss. Couceiro et al., COMPARATIVE-EVALUATION OF A SIMPLE AND SENSITIVE ASSAY FOR DETECTION OF ORTHOMYXO AND PARAMYXOVIRUSES, Memorias do Instituto Oswaldo Cruz, 89(2), 1994, pp. 183-187
Studies were done to evaluate comparatively the traditional HA assay a
nd a more recently introduced lectin-neuraminidase (LN) methodology in
search of a simple and sensitive assay for virus detection during lab
oratorial diagnosis. The results proved the value of LN assay as a sen
sitive Methodology for detection of virus particles, presenting result
s at least equal to those obtained by HA (hemagglutination) assay, wit
h significant values of accumulated frequencies for LN/HA factors (rat
ios between LN and HA titers) higher than two. The accumulated values
of frequencies for LN/HA factors as high as four were very significant
, 72.7% for influenzavirus and 60.7% for Newcastle disease virus (NDV)
, moreover accumulated frequencies for LN/HA factors even as high as 3
2 were due to influenzavirus (45.4%) and NDV (7.2%) samples. After the
storage period, most of those concentrated samples that even did not
present HA titers could be detected through LN assay, demonstrating a
lower threshold for virus detection.