GENOTYPE-INDEPENDENT TRANSFORMATION OF LETTUCE USING AGROBACTERIUM-TUMEFACIENS

Authors
CURTIS IS POWER JB BLACKHALL NW DELAAT AMM DAVEY MR
Citation
Is. Curtis et al., GENOTYPE-INDEPENDENT TRANSFORMATION OF LETTUCE USING AGROBACTERIUM-TUMEFACIENS, Journal of Experimental Botany, 45(279), 1994, pp. 1441-1449
Citations number
21
Categorie Soggetti
Plant Sciences
Journal title
Journal of Experimental Botany → ACNP
ISSN journal
00220957
Volume
45
Issue
279
Year of publication
1994
Pages
1441 - 1449
Database
ISI
SICI code
0022-0957(1994)45:279<1441:GTOLUA>2.0.ZU;2-0
Abstract
The genetic manipulation of lettuce (Lactuca sativa) necessitates a re liable and efficient, genotype-independent method of transformation. T hirteen lettuce cultivars have been assessed for their amenability to Agrobacterium-mediated gene transfer linked to their tissue culture re sponsiveness, including callus induction and shoot regeneration. A rel iable protocol has been developed for the routine production of transg enic plants for all 13 cultivars investigated.