PRODUCTION AND CHARACTERIZATION OF A NEW MONOCLONAL-ANTIBODY, GR-ICOR-2, RECOGNIZING SARCOMERIC ACTIN - ANALYSIS OF THE EXPRESSION IN THE DEVELOPING CHICK HEART

Authors
FERNANDEZ JE MELGUIZO C PRADOS J MARCHAL JA ALVAREZ L ARANEGA A
Citation
Je. Fernandez et al., PRODUCTION AND CHARACTERIZATION OF A NEW MONOCLONAL-ANTIBODY, GR-ICOR-2, RECOGNIZING SARCOMERIC ACTIN - ANALYSIS OF THE EXPRESSION IN THE DEVELOPING CHICK HEART, Histology and histopathology, 9(4), 1994, pp. 765-771
Citations number
34
Categorie Soggetti
Cytology & Histology
Journal title
Histology and histopathology → ACNP
ISSN journal
02133911
Volume
9
Issue
4
Year of publication
1994
Pages
765 - 771
Database
ISI
SICI code
0213-3911(1994)9:4<765:PACOAN>2.0.ZU;2-H
Abstract
We produced and characterized a specific monoclonal antibody (mAB) des ignated GR-ICOR-2. This mAb recognizes sarcomeric actin molecules (43 kDa) and was used in an immunohistochemical analysis of staining patte rns in Hamburger and Hamilton's stages 18, 22 and 25 (HK 18, 22 and 25 ) embryonic chick hearts. Staining showed a mainly cytoplasmic distrub ition in three regions: the atrioventricular (AV) canal cushion tissue , the primitive ventricle, and conal crests. In addition, this mAb-cro ss-reacted with rabbit and human cardiac and skeletal muscle tissue; b ut not with smooth muscle tissue.