DISULFIDE ARRANGEMENT IN BOVINE CASEINS - LOCALIZATION OF INTRACHAIN DISULFIDE BRIDGES IN MONOMERS OF KAPPA-CASEIN AND ALPHA(S2)-CASEIN FROM BOVINE-MILK

Authors
RASMUSSEN LK HOJRUP P PETERSEN TE
Citation
Lk. Rasmussen et al., DISULFIDE ARRANGEMENT IN BOVINE CASEINS - LOCALIZATION OF INTRACHAIN DISULFIDE BRIDGES IN MONOMERS OF KAPPA-CASEIN AND ALPHA(S2)-CASEIN FROM BOVINE-MILK, Journal of Dairy Research, 61(4), 1994, pp. 485-493
Citations number
26
Categorie Soggetti
Food Science & Tenology","Agriculture Dairy & AnumalScience
Journal title
Journal of Dairy Research → ACNP
ISSN journal
00220299
Volume
61
Issue
4
Year of publication
1994
Pages
485 - 493
Database
ISI
SICI code
0022-0299(1994)61:4<485:DAIBC->2.0.ZU;2-X
Abstract
Naturally occurring monomeric kappa-casein and alpha(s2)-casein in bov ine milk were purified by ion-exchange chromatography in order to loca lize potential intrachain disulphide bridges. Enzymic cleavage of the proteins followed by mass spectrometry and amino acid sequence analysi s of cystine-containing peptides revealed the presence of an intrachai n disulphide bond in both proteins.