EFFECT OF H2O2 ADDITION MODE ON ENZYMATIC REMOVAL OF PHENOL FROM WASTE-WATER IN THE PRESENCE OF POLYETHYLENE-GLYCOL

The effect of batch and semi-batch additions of the stoichiometric amo unt of H2O2 on enzymatic removal of phenol has been studied over the p henol concentration range of 1-10 mmol/L. It is found that the ratio b etween the maximum H2O2 concentration during the reaction and the init ial horseradish peroxidase concentration ((H2O2)(max)/(HRP)(0)) contro ls the rate of phenol removal by horseradish peroxidase in the presenc e of the additive, polyethylene glycol. An optimum range of this ratio is determined to be between 10 and 25 mu mol/U. Empirical models are proposed to predict the values of (H2O2)(max)/(HRP)(0) required to tre at given phenol concentrations under different modes for adding H2O2.