LIMITATIONS OF THE LINEAR SOLVATION ENERGY RELATIONSHIPS IN REVERSED-PHASE LIQUID-CHROMATOGRAPHY

We have re-examined the linear solvation energy relationships in rever sed phase liquid chromatography by considering various solutes includi ng quite a number of compounds of strong hydrogen bond capability. We observed that solutes of strong hydrogen bond ability should be exclud ed in order to obtain resonable correlations between In k' and solute polarity parameters and that inclusion of one or two such solutes caus es severe distortions of correlation results. This anomaly may be due to existence of residual silanol groups in the stationary phase, that is, their specific interactions with solutes.