Jp. Meng et al., CHARACTERIZATION AND DEVELOPMENTAL EXPRESSION OF A TESTIS-SPECIFIC ADENOSINE-DEAMINASE MESSENGER-RNA IN THE MOUSE, Journal of andrology, 18(1), 1997, pp. 88-95
Adenosine is able to alter intracellular cAMP levels and to affect the
physiological functions of sperm. It also modulates FSH action throug
h its Gi protein coupled receptors. Adenosine deaminase (ADA), an enzy
me regulating adenosine levels, exists in the plasma membrane of the s
perm, but little is known about its action on testicular function. The
present study investigates the expression of a testis-specific ADA mR
NA with development and in comparison to several other tissues in the
mouse. In addition, the sequence of a testis-specific ADA cDNA was det
ermined from an adult mouse testis library. We demonstrate for the fir
st time the presence of a major 1,350 bp testis-specific ADA mRNA and
a weaker 1,200 bp ADA transcript whose developmental expression starts
on day 28 of life. Sequence analysis of the testis-specific ADA cDNA
indicated that exons 1 and 2, as well as the first 8 nucleotides of ex
on 3 of the somatic cell ADA cDNA, were absent in the testicular ADA c
DNA. The deduced open reading frame of the testis-specific ADA cDNA in
dicates absence of the first 51 amino acids at the 5' end that are pre
sent in the somatic cell ADA protein. The developmental onset of expre
ssion of the testis-specific ADA mRNA may be related to specific proli
feration/differentiation events of spermatogenesis.