OPTIMIZATION OF LIPASE PRODUCTION BY PSEUDOMONAS-AERUGINOSA MB 5001 IN BATCH CULTIVATION

The production of Pseudomonas aeruginosa MB 5001 extracellular lipase was optimized by batch cultivation employing shake flasks and 23-L bio reactors. This enzyme efficiently and selectively bioconverts dimethyl oquinolin-2-yl)ethyl)phenyl)4,6-dithianonanedioate (diester) to its ( S)-ester acid. Proces development studies focused on the identificatio n and optimization of the physicochemical parameters required to achie ve maximum lipase production. Of the media evaluated, a peptonized mil k-based medium was found to support excellent lipase production and st ability. Medium composition and process parameters that supported opti mal lipase production were different from those supporting maximum bio mass formation. Of the parameters investigated, dissolved oxygen tensi on had the most significant and unexpected impact on lipase production . Elevated lipase production was achieved when P. aeruginosa MB 5001 w as cultivated in a dissolved oxygen limited environment. Overall, thes e process development studies resulted in a 100% increase in lipase pr oduction when compared to the original shake flask process employing s kim milk.