M. Bodzek et al., PREPARATION OF MEMBRANE-IMMOBILIZED ENZYMES FOR PHENOL DECOMPOSITION, Journal of chemical technology and biotechnology, 61(3), 1994, pp. 231-239
A method for the preparation of membranes with immobilised enzymes for
the biological degradation of phenols is described. In the first stag
e, an enzymatic fraction from bacterial strains has been isolated. The
enzyme membranes were obtained by ultrafiltration of protein solution
through a membrane support made of non-cellulose polymers. All enzyme
s were adsorbed onto the membrane surface. The enzyme membranes were f
ound to degrade 70-80% of the phenol during single dead-end ultrafiltr
ation at a pressure of 1 x 10(5)-3 x 10(5) Pa. The retention coefficie
nt of the ultrafiltration process also amounted to 70-80%.