MONOCLONAL-ANTIBODIES REACTING WITH THE MUCIN-SPECIFIC LECTIN FROM SAMBUCUS-SIEBOLDIANA (SSA-M)

Monoclonal antibodies (MAbs) were prepared against the mucin-specific lectin from Sambucus sieboldiana (SSA-M). The majority of MAbs reacted with both free SSA-M and SSA-M bound to porcine mucin. However, MAbs SS16, SS18, and SS19 did not react with mucin-bound lectin, suggesting that these MAbs may react at or near the lectin binding site. Some MA bs also showed reactivity with lectins other than SSA-M (e.g. ConA, Pi sum sativum, and Saphora japonica lectins) suggesting possible sequenc e homology between SSA-M and other lectins. These MAbs should be usefu l reagents for immunoaffinity purification of SSA-M, characterizing SS A-M structure and binding, and metabolic studies on mucins.