ALPHA-ANORDRIN-INDUCED APOPTOSIS OF LEUKEMIA K562 CELLS IS NOT PREVENTED BY CICLOHEXIMIDE

AIM: To study effect of protein synthesis inhibitor cicloheximide (Cic ) on the apoptosis induced by alpha-anordrin (Ano) in leukemia K562 ce lls. METHODS: Morphological changes were observed by fluorescent micro scopy. DNA content was measured by flow cytometry. DNA fragmentation W as analyzed by agarose gel electrophoresis. RESULTS: Exposure of K562 cells to Ano 50 mu mol . L(-1) for 24 h induced apoptotic cell death. Cic 1 mu mol . L(-1) did not abrogate or delay this effect. Indeed, An o-induced apoptosis Was augmented by Cic. Cic 100 mu mol . L(-1) itsel f stimulated 25 % K562 cell apoptosis after 24-h culture. CONCLUSION: Ano-induced apoptosis was independent of de now, protein synthesis.