CD-113-H-1 HETEROTOCSY - A METHOD FOR DETERMINING METAL-PROTEIN CONNECTIVITIES

Cd-113-H-1 NMR correlation experiments have been extremely useful for determining the amino acid ligands that form metal-binding sites in pr oteins. To date, the majority of these methods have used heteronuclear multiple-quantum transfer as the basis for establishing correlations. In this paper, we demonstrate the feasibility of using correlation me thods that employ heteronuclear cross-polarization (heteroTOCSY) as vi able alternatives. Additionally, we couple heteroTOCSY with selective excitation and transfer procedures to take advantage of the small numb er of heteronuclei usually present in metalloprotein systems. One- and two-dimensional experiments are presented as examples of these techni ques.